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Authoritative and up-to-date,Poly(ADP-Ribose) Polymerase: Methods and Protocols, Third Editionpresents essential new and classical methods for studying the pADPr-pathway.
Part I: Detection and Quantification of pADPr
1. Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High Performance Liquid Chromatography, and Capillary Electrophoresis
Mohsen Badiee, Audrey Boutonnet, Dat Phan, and Anthony K.L. Leung
2. Detecting and Quantifying pADPr In Vivo
Andrew M. Lamade, Yaming Chen, Carla J. Johnson, Hülya Bayır, and Robert S.B. Clark
3. Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage
Christopher A. Koczor, Kate M. Saville, Rasha Q. Al-Rahahleh, Joel F. Andrews, Jianfeng Li, and Robert W. Sobol
4. Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly(ADP-Ribose)
Johannes Rudolph and Karolin Luger
5. Detecting Poly(ADP-Ribose) In Vitro and in Cells Using PAR-Trackers
Sridevi Challa, Amy L. Whitaker, and W. Lee Kraus
6. An Enzyme-Linked Immunosorbent Assay to Quantify Poly(ADP-Ribose) Level In Vivo
Chieri Ida, Sachiko Yamashita, Takayuki Eguchi, Yasuhito Kuroda, Setsu Nakae, Yoshisuke Nishi, Kazuo Kamemura, Tsuyoshi Shirai, Tamio Mizukami, Masakazu Tanaka, Joel Moss, and Masanao Miwa
7. Subcellular Quantitation of ADP-Ribosylation by High Content Microscopy
Lukas Muskalla, Anka Güldenpfennig, and Michael O. Hottiger
8. A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery
Johannes Gregor Matthias Rack and Ivan Ahel
Part II: Identification of Protein Targets
9. Immunoprecipitation Using Mono-ADP-Ribosylation Specific Antibodies
Helen Dauben and Ivan Matic
10. A Clickable NAD+Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins
Dongsheng Yao, Heba Ahmed, and Junqi Song
11. Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells
Dan Huang, Andrea D. Edwards, Xuan Gong, and W. Lee Kraus
Part III: Functional Analysis
12. Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer
Nura Lutfi, Carlos Martínez, and José Yélamos
13. Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2), a Cation Channel Activated by ADP-Ribose and Involved in Cell Death
Hannah K. Hall and David W. Koh
14. Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function
Tünde Kovács, Boglárka Rauch, Edit Mikó, and Péter Bai
15. Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrometry
Holda A. Anagho, Jonas D. Elsborg, Ivo A. Hendriks, Sara C. Buch-Larsen, and Michael L. Nielsen
16. Cytological Approaches to Visualize Intracellular Dynamics of RNA Binding Proteins at Active Genes in Drosophila
Anand K. Singh and Subhash C. Lakhotia
Part IV: Poly(ADP-Ribosyl)ating Pathway in Chromatin and Gene Expression
17. Chromatin Immunoprecipitation Approach to Determine How PARP1 Domains Affect Binding Pattern to Chromatin
Guillaume Bordet, Gbolahan Bamgbose, Sayem H. Bhuiyam, Sarah Johnson, and Alexei V. Tulin
18. Approach to Measuring the Effect of PARP1 on RNA Polymerase II Elongation Rates
Hejer Dhahri, Elena Matveeva, and Yvonne Fondufe-Mittendorf
19. Examining the Effect of PARP-1 Inhibitors on Transcriptional Activity of Androgen Receptor in Prostate Cancer Cells
Peter Makhov, Rushaniya Fazliyeva, Antonio Tufano, Robert G. Uzzo, and Vladimir M. Kolenko
Part V: Using Animal Models
20. Using Drosophila Genetics to Identify Factors that Affect PARP1 Activity In Vivo
Guillaume Bordet and Alexei V. Tulin
21. Generating PARP KnockoutD. Melanogasterwith CRISPR/Cas9 System
Yaroslava Karpova and Alexei V. Tulin
22. TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice
Yaroslava Karpova and Alexei V. Tulin
Part VI: Designing and Testing PARP-1 Inhibitors
23. Cell-Based Screening for New PARP Inhibitors Utilizing PARG Mutated Mouse Embryonic Stem Cells
Yaroslava Karpova, Danping Guo, and Alexei V. Tulin
24. Quantification of PARP7 Protein Levels and PARP7 Inhibitor Target Engagement in Cells Using a Split Nanoluciferase System
Anna K. Duell, Daniel J. Sanderson, and Michael S. Cohen
Part VII: Targeting the Understudied Components of pADPr Pathway
25. Purification of Recombinant Human PARG and Activity Assays
Jean-Christophe Amé and Françoise Dantzer
26. Purification of Recombinant Human PARP-3
Jean-Christophe Amé, Leonel Nguekeu-Zebase, Daisy Harwood, Zuleyha Yildirim, Lisa Roegel, Agathe Boos, and Françoise Dantzer
Catégories
Caractéristiques
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- ISBN9781071628904
- Code produit258876
- ÉditeurHUMANA PRESS
- CollectionMethods in Molecular Biology
- Date de publication15 décembre 2022
- FormatPapier
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